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One study, for example, treated Sprague Dawley rats treated with gestational nicotine (3 mg/kg/day) and found that PNE offspring (P35/P36) had altered myelin expression of myelin-related transcription and trophic factors that produced alterations in myelin gene expression and oligodendrocyte functioning [165]

A549, RPMI2650, BEAS-2B, or MLE12 cells were seeded on 12 well-transwell inserts (Corning, ME, USA) for 24 h before CSE treatment and then, cells were cultured with 10% CSE-containing media for 48 h, rinsed with PBS

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